Immunotation of Rv0013
From DrugPedia: A Wikipedia for Drug discovery
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| PDB = | | PDB = | ||
| DrugBank = | | DrugBank = | ||
| - | | chemical_formula = | + | | chemical_formula = |
| molecular_weight = 24626.9 Da | | molecular_weight = 24626.9 Da | ||
| solubility = | | solubility = | ||
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=General= | =General= | ||
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Mb0013, trpG, len: 232 aa. Equivalent to Rv0013, len: 232 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 232 aa overlap). Possible trpG, anthranilate synthase component II (glutamine amidotransferase) (EC 4.1.3.27), equivalent to NP_301141.1|NC_002677 putative p-aminobenzoate synthase glutamine amidotransferase from Mycobacterium leprae (232 aa). Also highly similar to others e.g. P26922|TRPG_AZOBR Anthranilate synthase component II from Azospirillum brasilense (196 aa), FASTA scores: opt: 703, E(): 8.6e-40, (56.7% identity in 187 aa overlap); T36720 probable glutamine amidotransferase from Streptomyces coelicolor (212 aa); T44524 anthranilate synthase from Nitrosomonas europaea (199 aa); etc. Also similar to E235740 para-aminobenzoate synthase (232 aa), FASTA scores: opt: 1273, E(): 0, (79.7% identity in 232 aa overlap). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. SIMILARITY TO OTHER TYPE-1 GLUTAMINE AMIDOTRANSFERASE DOMAINS. Note that previously known as pabA. | Mb0013, trpG, len: 232 aa. Equivalent to Rv0013, len: 232 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 232 aa overlap). Possible trpG, anthranilate synthase component II (glutamine amidotransferase) (EC 4.1.3.27), equivalent to NP_301141.1|NC_002677 putative p-aminobenzoate synthase glutamine amidotransferase from Mycobacterium leprae (232 aa). Also highly similar to others e.g. P26922|TRPG_AZOBR Anthranilate synthase component II from Azospirillum brasilense (196 aa), FASTA scores: opt: 703, E(): 8.6e-40, (56.7% identity in 187 aa overlap); T36720 probable glutamine amidotransferase from Streptomyces coelicolor (212 aa); T44524 anthranilate synthase from Nitrosomonas europaea (199 aa); etc. Also similar to E235740 para-aminobenzoate synthase (232 aa), FASTA scores: opt: 1273, E(): 0, (79.7% identity in 232 aa overlap). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. SIMILARITY TO OTHER TYPE-1 GLUTAMINE AMIDOTRANSFERASE DOMAINS. Note that previously known as pabA. | ||
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<td>subject ids</td><td>% identity</td><td>% positives</td><td>alignment length</td><td> evalue</td></tr> | <td>subject ids</td><td>% identity</td><td>% positives</td><td>alignment length</td><td> evalue</td></tr> | ||
| - | <tr><td>sp|P27708.3|</td><td> | + | <tr><td>sp|P27708.3|</td><td> 29</td><td> 46</td><td> 132</td><td> 1e-08</td></tr> |
| + | <tr><td>sp|P49915.1|</td><td> 28</td><td> 44</td><td> 112</td><td> 2e-06</td></tr> | ||
| + | <tr><td>sp|P31327.2|</td><td> 27</td><td> 42</td><td> 137</td><td> 2e-05</td></tr> | ||
| + | <tr><td>sp|Q92820.2|</td><td> 24</td><td> 40</td><td> 123</td><td> 0.33 </td></tr> | ||
| + | <tr><td>sp|Q6UW60.1|</td><td> 28</td><td> 40</td><td> 75</td><td> 5.5</td></tr></table> | ||
| + | |||
| + | =Alergen Protein= | ||
| + | Link to [http://www.imtech.res.in/raghava/algpred/ Algpred]<br> | ||
| + | Non Allergen Predicted by AlgPred Server | ||
| + | |||
| + | =Bacterial Toxin Prediction= | ||
| + | Link to [http://www.imtech.res.in/raghava/btxpred/submission.html btxpred]<br> | ||
| + | No Hit Fountd by btxpred server. | ||
| + | |||
| + | =Subcellular Location= | ||
| + | Link to [http://www.imtech.res.in/raghava/tbpred/ TBpred]<br> | ||
| + | Cytoplasmic Protein | ||
| + | |||
| + | =Antigens= | ||
| + | No Hit Found in [http://www.imtech.res.in/raghava/antigendb/keyquery.html AntigenDB]<br> | ||
| + | No. Hit Found in [http://www.iedb.org/ IEDB] | ||
| + | |||
| + | ==B cell Epitopes== | ||
| + | ====BCEpred Analysis==== | ||
| + | Link to [http://www.imtech.res.in/raghava/bcepred/bcepred_submission.html Bcepred] | ||
| + | <br> | ||
| + | Result Predicited by[http://crdd.osdd.net/drugpedia/index.php/Image:Rv0013bcpred.doc] | ||
| + | |||
| + | ====ABCpred Analysis==== | ||
| + | Link to [http://www.imtech.res.in/raghava/abcpred/ABC_submission.html ABCpred]<br> | ||
| + | Result Predicted By | ||
| + | [http://crdd.osdd.net/drugpedia/index.php/Image:ABCpred_Prediction_ServerRv0013.doc]<br> | ||
| + | |||
| + | ====IEDB Analysis==== | ||
| + | Link to [http://tools.immuneepitope.org/tools/bcell/iedb_input IEDB]<br> | ||
| + | No Hits Found. | ||
| + | |||
| + | =MHC Class-I Binder= | ||
| + | ==NHLA Analysis== | ||
| + | Link to [http://www.imtech.res.in/raghava/nhlapred/comp.html]<br> | ||
| + | Results[http://crdd.osdd.net/drugpedia/index.php/Image:NHLAPredRv0013.doc]</br> | ||
| + | |||
| + | ==IEDB Analysis== | ||
| + | Link to [http://tools.immuneepitope.org/analyze/html/mhc_processing.html IEDB] <br> | ||
| + | Results[http://crdd.osdd.net/drugpedia/index.php/Image:IEDBRv0013.doc] | ||
| + | |||
| + | =MHC Class-II Binder= | ||
| + | ==Propred Analysis== | ||
| + | Link to [http://www.imtech.res.in/raghava/propred/ Propred]<br> | ||
| + | Results[http://crdd.osdd.net/drugpedia/index.php/Image:ProphedRv0013.doc] | ||
| + | |||
| + | ==NetMHC-II Analysis== | ||
| + | Link to [http://www.cbs.dtu.dk/services/NetMHCII/ NetMHC-II]<br> | ||
| + | Results[http://crdd.osdd.net/drugpedia/index.php/Image:NetMHCII_2_Rv0013.doc]<br> | ||
| + | |||
| + | =External Links= | ||
| + | * [http://www.biohealthbase.org/mycobacterium Database] of ''Mycobacterium tuberculosis'' genome sequences and related information. | ||
| + | |||
| + | [[Category:C2D]] | ||
| + | [[Category:Mtb Immunotation]] | ||
Current revision
| Immunotation of Rv0013
| |
| Name | |
| POSSIBLE ANTHRANILATE SYNTHASE COMPONENT II TRPG (GLUTAMINE AMIDOTRANSFERASE) | |
| Identifiers | |
| Swiss Prot | |
| Genbank | |
| PDB | ? |
| Chemical data | |
| Formula | ? |
| Mol. wt. | 24626.9 Da |
| Pharmacokinetic data | |
| Bioavailability | ? |
| Solubility | ? |
| Isoelectric-Point | 6.502 |
Contents |
[edit] General
Mb0013, trpG, len: 232 aa. Equivalent to Rv0013, len: 232 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 232 aa overlap). Possible trpG, anthranilate synthase component II (glutamine amidotransferase) (EC 4.1.3.27), equivalent to NP_301141.1|NC_002677 putative p-aminobenzoate synthase glutamine amidotransferase from Mycobacterium leprae (232 aa). Also highly similar to others e.g. P26922|TRPG_AZOBR Anthranilate synthase component II from Azospirillum brasilense (196 aa), FASTA scores: opt: 703, E(): 8.6e-40, (56.7% identity in 187 aa overlap); T36720 probable glutamine amidotransferase from Streptomyces coelicolor (212 aa); T44524 anthranilate synthase from Nitrosomonas europaea (199 aa); etc. Also similar to E235740 para-aminobenzoate synthase (232 aa), FASTA scores: opt: 1273, E(): 0, (79.7% identity in 232 aa overlap). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. SIMILARITY TO OTHER TYPE-1 GLUTAMINE AMIDOTRANSFERASE DOMAINS. Note that previously known as pabA.
MOLECULAR FUNTION
POSSIBLY INVOLVED IN BIOSYNTHESIS OF TRYPTOPHAN (AT THE FIRST STEP). SUPPOSED TETRAMER OF TWO COMPONENTS I AND TWO COMPONENTS II: COMPONENT I (Rv1609|trpE) CATALYZES THE FORMATION OF ANTHRANILATE USING AMMONIA RATHER THAN GLUTAMINE, WHEREAS COMPONENT II (Rv0013|trpG) PROVIDES GLUTAMINE AMIDOTRANSFERASE ACTIVITY. POSSIBLY PARTICIPATES IN THE TRYPTOPHAN-DEPENDENT INDOLE-3-ACETIC ACID PRODUCTION [CATALYTIC ACTIVITY: CHORISMATE + L-GLUTAMINE = ANTHRANILATE + PYRUVATE + L-GLUTAMATE].
[edit] Protein Sequence
>M. bovis AF2122/97|Mb0013|TrpG: 232 aa - POSSIBLE ANTHRANILATE SYNTHASE COMPONENT II TRPG (GLUTAMINE AMIDOTRANSFERASE)</b>
MRILVVDNYDSFVFNLVQYLGQLGIEAEVWRNDDHRLSDEA
AVAGQFDGVLLSPGPGTPERAGASVSMVHACAAAHTPLLGV
CLGHQAIGVAFGATVDRAPELLHGKTSSVFHTNVGVLQGLP
DPFTATRYHSLTILPKSLPAVLRVTARTSSGVIMAVQHTGL
PIHGVQFHPESILTEGGHRILANWLTCCGWTQDDTLVRRLE
NEVLTAISPHFPTSTASAGEATGRTSA
[edit] Human Homologue Blast Result
| subject ids | % identity | % positives | alignment length | evalue |
| sp|P27708.3| | 29 | 46 | 132 | 1e-08 |
| sp|P49915.1| | 28 | 44 | 112 | 2e-06 |
| sp|P31327.2| | 27 | 42 | 137 | 2e-05 |
| sp|Q92820.2| | 24 | 40 | 123 | 0.33 |
| sp|Q6UW60.1| | 28 | 40 | 75 | 5.5 |
[edit] Alergen Protein
Link to Algpred
Non Allergen Predicted by AlgPred Server
[edit] Bacterial Toxin Prediction
Link to btxpred
No Hit Fountd by btxpred server.
[edit] Subcellular Location
Link to TBpred
Cytoplasmic Protein
[edit] Antigens
No Hit Found in AntigenDB
No. Hit Found in IEDB
[edit] B cell Epitopes
[edit] BCEpred Analysis
Link to Bcepred
Result Predicited by[1]
[edit] ABCpred Analysis
Link to ABCpred
Result Predicted By
[2]
[edit] IEDB Analysis
Link to IEDB
No Hits Found.
[edit] MHC Class-I Binder
[edit] NHLA Analysis
[edit] IEDB Analysis
[edit] MHC Class-II Binder
[edit] Propred Analysis
[edit] NetMHC-II Analysis
[edit] External Links
- Database of Mycobacterium tuberculosis genome sequences and related information.
