PolysacDB 2349
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[edit] Carbohydrate Name
K antigens [Capsular polysaccharide]
[edit] Carbohydrate Class
Capsular polysaccharide
[edit] Source Microbe
Sinorhizobium meliloti strain NRG185
[edit] Basic Structure
The K antigens of Sinorhizobium spp. are major strain-specific antigens, which commonly consist of small repeating units of a hexose and 1-carboxy-2-keto-3-deoxy sugars, such as sialic acid or 3-deoxy-D-manno-2-octulosonic acid (Kdo), although they vary in glycosyl residue composition, substitution patterns, linkage points, anomeric configuration, and size range. The K-antigens of rhizobia do not possess a lipid anchor
[edit] Proposed functions
K antigens may be involved in an early recognition step in S. meliloti-alfalfa interactions and that K antigen production is affected by plant-derived signals
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab 8
[edit] Antibody type and class
IgGM
[edit] Assay System
ELISA and immunoblotting
[edit] Cross-reactivity
This Mab was specific to S. melliloti. But MAb 8 recognized the K antigens from both the homologous strain, S. meliloti NRG185, and S. meliloti NRG286
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
The use of MAbs to study specific cell surface components from the bacteroids of Rhizobium leguminosarum have yielded interesting information about morphogenesis in the pea symbiont, including the temporal expression of specific epitopes during nodule development
