PolysacDB 2496
From DrugPedia: A Wikipedia for Drug discovery
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[edit] Carbohydrate Name
O-polysaccharide [Terminal disaccharide]
[edit] Carbohydrate Class
O-polysaccharide [LPS]
[edit] Source Microbe
Vibrio cholerae O1 LPS serotype Inaba
[edit] Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
[edit] Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
BSA
[edit] Conjugation Method
The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
[edit] Antibodies
Antsera
[edit] Antibody type and class
IgM and IgG1
[edit] Assay System
ELISA
[edit] Cross-reactivity
This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
This antisera was neither vibriocidal nor protective in the infant mouse cholera model
