PolysacDB Lipid-A 2574

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Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Lipid-A

[edit] Carbohydrate Class

Lipopolysaccharide

[edit] Source Microbe

Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety

[edit] Basic Structure

Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall

[edit] Proposed functions

Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment

[edit] Antigenic Nature used to produce antibodies

Whole cells

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab JIM32

[edit] Antibody type and class

IgG2b

[edit] Assay System

dot immunoassay, immunoblotting

[edit] Cross-reactivity

This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3. JIM 32 reacted positively with the LPS from many strains of Rhizobium tested (excluding the Rhizobium meliloti subgroup). JIM 32 did not react with representative strains from Bradyrhizobium, Azorhizobium or other related bacterial species

[edit] Proposed epitopes

galacturonic acid residues seemed to be a part of the immunodominanat region

[edit] Proposed Utility

This Mab could be useful for the elucidation of the structure and biosynthesis of LPS

[edit] Weblink

PubMed Central

[edit] External Links

• Link to PolysacDB database