Metagenomics
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Metagenomics is described as “the comprehensive study of nucleotide sequence, structure, regulation, and function”. Scientists can study the smallest component of an environmental system by extracting DNA from organisms in the system and inserting it into a model organism. The model organism then expresses this DNA where it can be studied using standard laboratory techniques. | Metagenomics is described as “the comprehensive study of nucleotide sequence, structure, regulation, and function”. Scientists can study the smallest component of an environmental system by extracting DNA from organisms in the system and inserting it into a model organism. The model organism then expresses this DNA where it can be studied using standard laboratory techniques. | ||
+ | |||
+ | ==Procedure of Metagenomics== | ||
+ | Metagenomics is employed as a means of systematically investigating, classifying, and manipulating the entire genetic material isolated from environmental samples. This is a multi-step process that relies on the efficiency of four main steps. The procedure consists of (i) the isolation of genetic material, (ii) manipulation of the genetic material, (iii) library construction, and the (iv) the analysis of genetic material in the metagenomic library. | ||
+ | |||
+ | The first step of the procedure is the isolation of the DNA. First, a sample is collected that represents the environment under investigation because the biological diversity will be different in different environments. The samples contain many different types of microorganism, the cells of which can be broken open using chemical methods such as alkaline conditions or physical methods such as sonication. Once the DNA from the cells is free, it must be separated from the rest of the sample. This is accomplished by taking advantage of the physical and chemical properties of DNA. Some methods of DNA isolation include density centrifugation, affinity binding, and solubility/precipitation. | ||
+ | |||
+ | Once the DNA is collected, it is manipulated so that it can be used in the model organism. Genomic DNA (the genetic material of an organism) is relatively large so it is cut up into smaller fragments using enzymes called restriction endonucleases. These are special enzymes that cut DNA at a particular sequence of base pairs. The enzymes move along the long fragments until they recognize these sequences where they cut both strands of the DNA. This results in the smaller, linear fragments of DNA depicted. The fragments are then combined with vectors. Vectors are small units of DNA that can be inserted into cells where they can replicate and produce the proteins encoded on the DNA using the machinery that the cells use to express normal genes. The vectors also contain a selectable marker. Selectable markers provide a growth advantage that the model organism would not normally have (such as resistance to a particular antibiotic) and are used to identify which organisms contain vectors and which ones do not. | ||
+ | |||
+ | The third step is to introduce the vectors with the metagenomic DNA fragments into the model organism. This allows the DNA from organisms that would not grow under laboratory conditions to be grown, expressed, and studied. The DNA inserted in the vector is transformed into cells of a model organism, typically Escherichia coli. Transformation is the physical insertion of foreign DNA into a cell, followed by stable expression of proteins. It can be done by chemical, electrical, or biological methods. The method of transformation is determined based on the type of sample used and the required efficiency of the reaction. The metagenomic DNA in the vectors are all in the same sample initially but the vectors are designed so that only one kind of DNA fragment from the sample will be maintained in each individual cell. The transformed cells are then grown on selective media so that only the cells carrying vectors will survive. Each group of cells that grows is called a colony. Each colony consists of many cloned cells that originated from one single cell. These samples of cells containing all of the metagenomic DNA samples on vectors are called metagenomic libraries. Each colony can be used to create a stock of cells for future study of a single fragment of the DNA from the environmental sample. | ||
+ | |||
+ | The fourth and final step in the procedure is the analysis of the DNA from the metagenomic libraries. The expression of DNA determines the physical and chemical properties of organisms so there are many potential methods of analysis. A phenotype is the physical attribute associated with expression of a gene. An example of metagenomic analysis would be to look for an unusual colour or shape in the model organism. An aspect of the phenotype that is not readily observed is chemical reaction. The chemical properties of the expressed metagenomic DNA can be examined by performing chemical assay on products created by the model organism. This would investigate whether the model organism gained an enzymatic function that it was previously lacking such as use of an unusual nutrient source for growth under conditions that limit normal nutrient availability. | ||
+ | |||
+ | ==Metagenomic Library== | ||
+ | Metagenomic libraries are typically used to search for new forms of a known gene. First, the metagenomic DNA is inserted into a model organism that lacks a specific gene function. Restoration of a physical or chemical phenotype can then be used to detect genes of interest. A genotype is the specific sequence of the DNA and provides another means of analyzing the metagenomic DNA fragment. The sequence of the bases in the DNA can be compared to databases of known DNA to get information regarding the structure and organization of the metagenomic DNA. Comparisons of these sequences can provide insight into how the gene products (proteins) function. | ||
+ | |||
+ | ==Metagenomic Analysis== | ||
+ | Genotypic analysis is usually performed after phenotypic analysis. A typical metagenomic analysis involves several subsequent rounds of the procedure in order to definitively isolate target genes from environmental samples and to effectively characterize the information encoded by the DNA sequence. The information gained from the metagenomic procedure provides information regarding the structure, organization, evolution, and origin of the DNA and can be used in scientific applications for the benefit of society and the environment. | ||
+ | |||
+ | ==Sequence-Based Analysis== | ||
+ | Sequenced-based analysis can involve complete sequencing of clones containing phylogenetic anchors that indicate the taxonomic group that is the probable source of the DNA fragment. Alternatively, random sequencing can be conducted, and once a gene of interest is identified, phylogenetic anchors can be sought in the flanking DNA to provide a link of phylogeny with the functional gene. Sequence analysis guided by the identification of phylogenetic markers is a powerful approach first proposed by the DeLong group, which produced the first genomic sequence linked to a 16S rRNA gene of an uncultured archaeon (136). Subsequently, they identified an insert from seawater bacteria containing a 16S rRNA gene that affiliated with the -Proteobacteria. The sequence of flanking DNA revealed a bacteriorhodopsin-like gene. Its gene product was | ||
+ | shown to be an authentic photoreceptor, leading to the insight that bacteriorhodopsin genes are not limited to Archaea but are in fact abundant among the Proteobacteria of the ocean. | ||
+ | |||
+ | ==Functional Metagenomics== | ||
+ | Heterologous expression. A powerful yet challenging approach to metagenomic analysis is to identify clones that express a function. Success requires faithful transcription and translation of the gene or genes of interest and secretion of the gene product, if the screen or assay requires it to be extracellular. Functional analysis has identified novel antibiotics, antibiotic resistance genes,Na(Li)/H transporters, and degradative enzymes. The power of the approach is that it does not require that the genes of interest be recognizable by sequence analysis, | ||
+ | making it the only approach to metagenomics that has the potential to identify entirely new classes of genes for new or | ||
+ | known functions. The significant limitation is that many genes,perhaps most, will not be expressed in any particular host bacterium selected for cloning. In fact, there is an inherent contradiction in this approach—genes are cloned from exotic organisms to discover new motifs in biology, and yet these genes are required to be expressed in Escherichia coli or another domesticated bacterium in order to be detected. The | ||
+ | diversity of the organisms whose DNA has been successfully expressed in E. coli is surprising, but heterologous expression remains a barrier to extracting the maximum information from functional metagenomics analyses. | ||
+ | |||
+ | ==Applications of Metagenomics== | ||
+ | Many microorganisms have the ability to degrade waste products, make new drugs for medicine, make environmentally friendly plastics, or even make some of the ingredients of food we eat. By isolating the DNA from these organisms, it provides us with the opportunity to optimize these processes and adapt them for use in our society. As a result of ineffective standard laboratory culture techniques, the potential wealth of biological resources in nature (like microbes) is relatively untapped, unknown, and uncharacterized. Metagenomics represents a powerful tool to access the abounding biodiversity of native environmental samples. The valuable property of metagenomics is that it provides the capacity to effectively characterize the genetic diversity present in samples regardless of the availability of laboratory culturing techniques. Information from metagenomic libraries has the ability to enrich the knowledge and applications of many aspects of industry, therapeutics, and environmental sustainability. This information can then be applied to society in an effort to create a healthy human population that lives in balance with the environment. Metagenomics is a new and exciting field of molecular biology that is likely to grow into a standard technique for understanding biological diversity. | ||
+ | |||
+ | ==References== | ||
+ | 1.Kimball J. Kimball’s Biology Pages: Taxonomy. | ||
+ | |||
+ | 2. Jasper S. University of Texas: Life Sciences. | ||
+ | |||
+ | 3. Vogel TM, Nalin R (2003). Sequencing the metagenome. ASM News 69(3):107. | ||
+ | |||
+ | 4.Black, C., J. A. M. Fyfe, and J. K. Davies. 1995. A promoter associated with | ||
+ | the neisserial repeat can be used to transcribe the uvrB gene from Neisseria | ||
+ | gonorrhoeae. J. Bacteriol. 177:1952–1958. | ||
+ | |||
+ | 5.Cha´vez, S., J. C. Reyes, F. Cahuvat, F. J. Florencio, and P. Candau. 1995. | ||
+ | The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis | ||
+ | 6803: cloning, transcriptional analysis and disruption of the gdhA gene. | ||
+ | Plant Mol. Biol. 28:173–188. | ||
+ | |||
+ | 6.Courtois, S., C. M. Cappellano, M. Ball, F. X. Francou, P. Normand, G. | ||
+ | Helynck, A. Martinez, S. J. Kolvek, J. Hopke, M. S. Osburne, P. R. August, | ||
+ | R. Nalin, M. Guerineau, P. Jeannin, P. Simonet, and J. L. Pernodet. 2003. | ||
+ | Recombinant environmental libraries provide access to microbial diversity | ||
+ | for drug discovery from natural products. Appl. Environ. Microbiol. 69: | ||
+ | 49–55. | ||
+ | |||
+ | 7.Ding, M. a. D. B. Y. 1993. Cloning and analysis of the leuB gene of | ||
+ | Leptospira interrogans serovar pomona. J. Gen. Microbiol. 139:1093–1103. | ||
+ | |||
+ | 8.Ferreyra, R. G., F. C. Soncini, and A. M. Viale. 1993. Cloning, characterization, | ||
+ | and functional expression in Escherichia coli of chaperonin | ||
+ | (groESL) genes from the prototrophic sulfur bacterium Chromatium vinosum. | ||
+ | J. Bacteriol. 175:1514–1523. | ||
+ | |||
+ | 9.Gillespie, D. E., S. F. Brady, A. D. Bettermann, N. P. Cianciotto, M. R. | ||
+ | Liles, M. R. Rondon, J. Clardy, R. M. Goodman, and J. Handelsman. 2002. | ||
+ | Isolation of antibiotics turbomycin A and B from a metagenomic library of | ||
+ | soil microbial DNA. Appl. Environ. Microbiol. 68:4301–4306. | ||
+ | |||
+ | 10.MacNeil, I. A., C. L. Tiong, C. Minor, P. R. August, T. H. Grossman, K. A. | ||
+ | Loiacono, B. A. Lynch, T. Phillips, S. Narula, R. Sundaramoorthi, A. Tyler, | ||
+ | T. Aldredge, H. Long, M. Gilman, D. Holt, and M. S. Osburne. 2001. | ||
+ | Expression and isolation of antimicrobial small molecules from soil DNA | ||
+ | libraries. J. Mol. Microbiol. Biotechnol. 3:301–308. | ||
+ | |||
+ | 11.Quaiser, A., T. Ochsenreiter, C. Lanz, S. C. Schuster, A. H. Treusch, J. Eck, | ||
+ | and C. Schleper. 2003. Acidobacteria form a coherent but highly diverse | ||
+ | group within the bacterial domain: evidence from environmental genomics. | ||
+ | Mol. Microbiol. 50:563–575. |
Current revision
Metagenomics is described as “the comprehensive study of nucleotide sequence, structure, regulation, and function”. Scientists can study the smallest component of an environmental system by extracting DNA from organisms in the system and inserting it into a model organism. The model organism then expresses this DNA where it can be studied using standard laboratory techniques.
Contents |
[edit] Procedure of Metagenomics
Metagenomics is employed as a means of systematically investigating, classifying, and manipulating the entire genetic material isolated from environmental samples. This is a multi-step process that relies on the efficiency of four main steps. The procedure consists of (i) the isolation of genetic material, (ii) manipulation of the genetic material, (iii) library construction, and the (iv) the analysis of genetic material in the metagenomic library.
The first step of the procedure is the isolation of the DNA. First, a sample is collected that represents the environment under investigation because the biological diversity will be different in different environments. The samples contain many different types of microorganism, the cells of which can be broken open using chemical methods such as alkaline conditions or physical methods such as sonication. Once the DNA from the cells is free, it must be separated from the rest of the sample. This is accomplished by taking advantage of the physical and chemical properties of DNA. Some methods of DNA isolation include density centrifugation, affinity binding, and solubility/precipitation.
Once the DNA is collected, it is manipulated so that it can be used in the model organism. Genomic DNA (the genetic material of an organism) is relatively large so it is cut up into smaller fragments using enzymes called restriction endonucleases. These are special enzymes that cut DNA at a particular sequence of base pairs. The enzymes move along the long fragments until they recognize these sequences where they cut both strands of the DNA. This results in the smaller, linear fragments of DNA depicted. The fragments are then combined with vectors. Vectors are small units of DNA that can be inserted into cells where they can replicate and produce the proteins encoded on the DNA using the machinery that the cells use to express normal genes. The vectors also contain a selectable marker. Selectable markers provide a growth advantage that the model organism would not normally have (such as resistance to a particular antibiotic) and are used to identify which organisms contain vectors and which ones do not.
The third step is to introduce the vectors with the metagenomic DNA fragments into the model organism. This allows the DNA from organisms that would not grow under laboratory conditions to be grown, expressed, and studied. The DNA inserted in the vector is transformed into cells of a model organism, typically Escherichia coli. Transformation is the physical insertion of foreign DNA into a cell, followed by stable expression of proteins. It can be done by chemical, electrical, or biological methods. The method of transformation is determined based on the type of sample used and the required efficiency of the reaction. The metagenomic DNA in the vectors are all in the same sample initially but the vectors are designed so that only one kind of DNA fragment from the sample will be maintained in each individual cell. The transformed cells are then grown on selective media so that only the cells carrying vectors will survive. Each group of cells that grows is called a colony. Each colony consists of many cloned cells that originated from one single cell. These samples of cells containing all of the metagenomic DNA samples on vectors are called metagenomic libraries. Each colony can be used to create a stock of cells for future study of a single fragment of the DNA from the environmental sample.
The fourth and final step in the procedure is the analysis of the DNA from the metagenomic libraries. The expression of DNA determines the physical and chemical properties of organisms so there are many potential methods of analysis. A phenotype is the physical attribute associated with expression of a gene. An example of metagenomic analysis would be to look for an unusual colour or shape in the model organism. An aspect of the phenotype that is not readily observed is chemical reaction. The chemical properties of the expressed metagenomic DNA can be examined by performing chemical assay on products created by the model organism. This would investigate whether the model organism gained an enzymatic function that it was previously lacking such as use of an unusual nutrient source for growth under conditions that limit normal nutrient availability.
[edit] Metagenomic Library
Metagenomic libraries are typically used to search for new forms of a known gene. First, the metagenomic DNA is inserted into a model organism that lacks a specific gene function. Restoration of a physical or chemical phenotype can then be used to detect genes of interest. A genotype is the specific sequence of the DNA and provides another means of analyzing the metagenomic DNA fragment. The sequence of the bases in the DNA can be compared to databases of known DNA to get information regarding the structure and organization of the metagenomic DNA. Comparisons of these sequences can provide insight into how the gene products (proteins) function.
[edit] Metagenomic Analysis
Genotypic analysis is usually performed after phenotypic analysis. A typical metagenomic analysis involves several subsequent rounds of the procedure in order to definitively isolate target genes from environmental samples and to effectively characterize the information encoded by the DNA sequence. The information gained from the metagenomic procedure provides information regarding the structure, organization, evolution, and origin of the DNA and can be used in scientific applications for the benefit of society and the environment.
[edit] Sequence-Based Analysis
Sequenced-based analysis can involve complete sequencing of clones containing phylogenetic anchors that indicate the taxonomic group that is the probable source of the DNA fragment. Alternatively, random sequencing can be conducted, and once a gene of interest is identified, phylogenetic anchors can be sought in the flanking DNA to provide a link of phylogeny with the functional gene. Sequence analysis guided by the identification of phylogenetic markers is a powerful approach first proposed by the DeLong group, which produced the first genomic sequence linked to a 16S rRNA gene of an uncultured archaeon (136). Subsequently, they identified an insert from seawater bacteria containing a 16S rRNA gene that affiliated with the -Proteobacteria. The sequence of flanking DNA revealed a bacteriorhodopsin-like gene. Its gene product was shown to be an authentic photoreceptor, leading to the insight that bacteriorhodopsin genes are not limited to Archaea but are in fact abundant among the Proteobacteria of the ocean.
[edit] Functional Metagenomics
Heterologous expression. A powerful yet challenging approach to metagenomic analysis is to identify clones that express a function. Success requires faithful transcription and translation of the gene or genes of interest and secretion of the gene product, if the screen or assay requires it to be extracellular. Functional analysis has identified novel antibiotics, antibiotic resistance genes,Na(Li)/H transporters, and degradative enzymes. The power of the approach is that it does not require that the genes of interest be recognizable by sequence analysis, making it the only approach to metagenomics that has the potential to identify entirely new classes of genes for new or known functions. The significant limitation is that many genes,perhaps most, will not be expressed in any particular host bacterium selected for cloning. In fact, there is an inherent contradiction in this approach—genes are cloned from exotic organisms to discover new motifs in biology, and yet these genes are required to be expressed in Escherichia coli or another domesticated bacterium in order to be detected. The diversity of the organisms whose DNA has been successfully expressed in E. coli is surprising, but heterologous expression remains a barrier to extracting the maximum information from functional metagenomics analyses.
[edit] Applications of Metagenomics
Many microorganisms have the ability to degrade waste products, make new drugs for medicine, make environmentally friendly plastics, or even make some of the ingredients of food we eat. By isolating the DNA from these organisms, it provides us with the opportunity to optimize these processes and adapt them for use in our society. As a result of ineffective standard laboratory culture techniques, the potential wealth of biological resources in nature (like microbes) is relatively untapped, unknown, and uncharacterized. Metagenomics represents a powerful tool to access the abounding biodiversity of native environmental samples. The valuable property of metagenomics is that it provides the capacity to effectively characterize the genetic diversity present in samples regardless of the availability of laboratory culturing techniques. Information from metagenomic libraries has the ability to enrich the knowledge and applications of many aspects of industry, therapeutics, and environmental sustainability. This information can then be applied to society in an effort to create a healthy human population that lives in balance with the environment. Metagenomics is a new and exciting field of molecular biology that is likely to grow into a standard technique for understanding biological diversity.
[edit] References
1.Kimball J. Kimball’s Biology Pages: Taxonomy.
2. Jasper S. University of Texas: Life Sciences.
3. Vogel TM, Nalin R (2003). Sequencing the metagenome. ASM News 69(3):107.
4.Black, C., J. A. M. Fyfe, and J. K. Davies. 1995. A promoter associated with the neisserial repeat can be used to transcribe the uvrB gene from Neisseria gonorrhoeae. J. Bacteriol. 177:1952–1958.
5.Cha´vez, S., J. C. Reyes, F. Cahuvat, F. J. Florencio, and P. Candau. 1995. The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis 6803: cloning, transcriptional analysis and disruption of the gdhA gene. Plant Mol. Biol. 28:173–188.
6.Courtois, S., C. M. Cappellano, M. Ball, F. X. Francou, P. Normand, G. Helynck, A. Martinez, S. J. Kolvek, J. Hopke, M. S. Osburne, P. R. August, R. Nalin, M. Guerineau, P. Jeannin, P. Simonet, and J. L. Pernodet. 2003. Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products. Appl. Environ. Microbiol. 69: 49–55.
7.Ding, M. a. D. B. Y. 1993. Cloning and analysis of the leuB gene of Leptospira interrogans serovar pomona. J. Gen. Microbiol. 139:1093–1103.
8.Ferreyra, R. G., F. C. Soncini, and A. M. Viale. 1993. Cloning, characterization, and functional expression in Escherichia coli of chaperonin (groESL) genes from the prototrophic sulfur bacterium Chromatium vinosum. J. Bacteriol. 175:1514–1523.
9.Gillespie, D. E., S. F. Brady, A. D. Bettermann, N. P. Cianciotto, M. R. Liles, M. R. Rondon, J. Clardy, R. M. Goodman, and J. Handelsman. 2002. Isolation of antibiotics turbomycin A and B from a metagenomic library of soil microbial DNA. Appl. Environ. Microbiol. 68:4301–4306.
10.MacNeil, I. A., C. L. Tiong, C. Minor, P. R. August, T. H. Grossman, K. A. Loiacono, B. A. Lynch, T. Phillips, S. Narula, R. Sundaramoorthi, A. Tyler, T. Aldredge, H. Long, M. Gilman, D. Holt, and M. S. Osburne. 2001. Expression and isolation of antimicrobial small molecules from soil DNA libraries. J. Mol. Microbiol. Biotechnol. 3:301–308.
11.Quaiser, A., T. Ochsenreiter, C. Lanz, S. C. Schuster, A. H. Treusch, J. Eck, and C. Schleper. 2003. Acidobacteria form a coherent but highly diverse group within the bacterial domain: evidence from environmental genomics. Mol. Microbiol. 50:563–575.