PolysacDB Capsular polysaccharide 1012
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[edit] Carbohydrate Name
Capsular polysaccharide
[edit] Carbohydrate Class
Capsular polysaccharide
[edit] Source Microbe
Streptococci Group B
[edit] Basic Structure
A basic backbone of the following residues: -3-β-D-Galp-(1-->4) -β-D-Glcp[branched to 1-->4-D-Glp-α-D-NeuNACp]-(1-->6)-β-D-GlcNAcp-1-
[edit] Proposed functions
Invades the blood stream and multiply. This property of invasiveness is related to the anti-phagocytic properties conferred by its Capsular polysaccharide
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Tetanus toxoid
[edit] Conjugation Method
Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4C in a pH stat. Adipic acid dihydrazide [AH] was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. After tumbling for 18 h at 3 to 8C, the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8C and passed through a 4B-CL Sepharose column. The polysaccharide-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and tetanus toxoid [TT} per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M, and the pH was maintained at 5.6 with 0.1 N NaOH for 3 h at room temperature. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8C and was passed through a 4B-CL Sepharose column (5 by 95 cm) equilibrated in 0.2 M NaCl. The void volume fractions were stored in 0.01% thimerosal at 3 to 8C
[edit] Antibodies
Polysera
[edit] Antibody type and class
mainly IgG, particularly IgG1 and IgG3
[edit] Assay System
Double immunodiffusion, capillary precipitation, ELISA
[edit] Cross-reactivity
N/A
[edit] Proposed epitopes
N\A
[edit] Proposed Utility
Antibodies elicited by the conjugates had in vitroopsonic activities proposed to be a correlate of protective immunity
