PolysacDB Glucan 1014
From DrugPedia: A Wikipedia for Drug discovery
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[edit] Carbohydrate Name
Glucan
[edit] Carbohydrate Class
Miscellaneous
[edit] Source Microbe
Candida albicans
[edit] Basic Structure
Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
[edit] Proposed functions
Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Diphtheria toxoid CRM 197
[edit] Conjugation Method
A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices
[edit] Antibodies
Mab 2G8
[edit] Antibody type and class
IgG2
[edit] Assay System
Flow Cytometry, confocal microscopy, phospholipase assay, yeast growth inhibition assay
[edit] Cross-reactivity
This antibody binds both C. neoformans and Candida albicans
[edit] Proposed epitopes
N\A
[edit] Proposed Utility
MAb 2G8 inhibits the in vitro growth of C. neoformans and also exerts anti-C. neoformans protective effects in vivo.
