Fluorescence Loss In Photobleaching
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(New page: In Fluorescence Loss In Photobleaching (FLIP) experiment, a fluorescent cell is repeatedly photobleached within a small region while the whole cell is continuously imaged. Any regions of t...)
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In Fluorescence Loss In Photobleaching (FLIP) experiment, a fluorescent cell is repeatedly photobleached within a small region while the whole cell is continuously imaged. Any regions of the cell that are connected to the area being bleached will gradually lose fluorescence due to lateral movement of mobile proteins into this area. By contrast, the fluorescence in unconnected regions will not be affected.