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Revision as of 17:51, 14 February 2010

Immunotation of Rv0013
Name
POSSIBLE ANTHRANILATE SYNTHASE COMPONENT II TRPG (GLUTAMINE AMIDOTRANSFERASE)
Identifiers
Swiss Prot	Q7DAK6
Genbank	885955
PDB	?
Chemical data
Formula	[1]
Mol. wt.	24626.9 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	6.502

General

Mb0013, trpG, len: 232 aa. Equivalent to Rv0013, len: 232 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 232 aa overlap). Possible trpG, anthranilate synthase component II (glutamine amidotransferase) (EC 4.1.3.27), equivalent to NP_301141.1|NC_002677 putative p-aminobenzoate synthase glutamine amidotransferase from Mycobacterium leprae (232 aa). Also highly similar to others e.g. P26922|TRPG_AZOBR Anthranilate synthase component II from Azospirillum brasilense (196 aa), FASTA scores: opt: 703, E(): 8.6e-40, (56.7% identity in 187 aa overlap); T36720 probable glutamine amidotransferase from Streptomyces coelicolor (212 aa); T44524 anthranilate synthase from Nitrosomonas europaea (199 aa); etc. Also similar to E235740 para-aminobenzoate synthase (232 aa), FASTA scores: opt: 1273, E(): 0, (79.7% identity in 232 aa overlap). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. SIMILARITY TO OTHER TYPE-1 GLUTAMINE AMIDOTRANSFERASE DOMAINS. Note that previously known as pabA. MOLECULAR FUNTION

POSSIBLY INVOLVED IN BIOSYNTHESIS OF TRYPTOPHAN (AT THE FIRST STEP). SUPPOSED TETRAMER OF TWO COMPONENTS I AND TWO COMPONENTS II: COMPONENT I (Rv1609|trpE) CATALYZES THE FORMATION OF ANTHRANILATE USING AMMONIA RATHER THAN GLUTAMINE, WHEREAS COMPONENT II (Rv0013|trpG) PROVIDES GLUTAMINE AMIDOTRANSFERASE ACTIVITY. POSSIBLY PARTICIPATES IN THE TRYPTOPHAN-DEPENDENT INDOLE-3-ACETIC ACID PRODUCTION [CATALYTIC ACTIVITY: CHORISMATE + L-GLUTAMINE = ANTHRANILATE + PYRUVATE + L-GLUTAMATE].

Protein Sequence

>M. bovis AF2122/97|Mb0013|TrpG: 232 aa - POSSIBLE ANTHRANILATE SYNTHASE COMPONENT II TRPG (GLUTAMINE AMIDOTRANSFERASE)</b>

MRILVVDNYDSFVFNLVQYLGQLGIEAEVWRNDDHRLSDEA AVAGQFDGVLLSPGPGTPERAGASVSMVHACAAAHTPLLGV CLGHQAIGVAFGATVDRAPELLHGKTSSVFHTNVGVLQGLP DPFTATRYHSLTILPKSLPAVLRVTARTSSGVIMAVQHTGL PIHGVQFHPESILTEGGHRILANWLTCCGWTQDDTLVRRLE NEVLTAISPHFPTSTASAGEATGRTSA

Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp|P27708.3|	84	61	132	1e-08