CarboDB Lipopolysaccharide 1900
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 00:00, 15 January 2001
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Pasteurella haemolytica serotype 1
Basic Structure
LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides. The O-antigen part consists of the following residues : -3)-β-D-Galp-(1-->3)-β-D-GalpNAc-(1-->4)-β-D-Galp-(1-. The core oligosaccharide part consists of the following residues : β-D-Galp-(1-->7)-D-gro-α-D-manHepp-(1-->6)-D-gro-α-D-manHepp-(1-->6)-β-D-Glcp-(1-->4)-L-gro [branched to L-gro-α-D-manHepp-(1-->2)-L-gro-α-D-manHepp-(1-->3)] and [branched to α-D-Glcp-(1-->6)] -α-D-manHepp-(1--/lipid A
Proposed functions
LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodies
P. hemolytica extract
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab ElF10
Antibody type and class
IgG3
Assay System
ELISA, immunoblotting
Cross-reactivity
This MAb had intense cross-reactivity among P. haemolytica serotypes 1, 5, 6, 7, 8, and 12 and less reactivity with serotypes 4 and 14 but did not react with LPS from any other gram negative bacteria tested
Proposed epitopes
N/A
Proposed Utility
This MAb to the LPS of P. haemolytica could be used to help further characterize the role of LPS in the pneumonic pasteurellosis disease process, including common binding sites and epitopes within the LPS molecule. It could be used in a screening kit, such as latex bead agglutination, to rapidly determine the presence of P. haemolytica in pneumonic lesions
