CarboDB Tetrasaccharide 2001

From DrugPedia: A Wikipedia for Drug discovery

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Revision as of 00:00, 15 January 2001

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Tetrasaccharide

Carbohydrate Class

Capsular polysaccharide

Source Microbe

Streptococcus pneumoniae serotype 6B

Basic Structure

The structure of the trisaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]

Proposed functions

N/A

Antigenic Nature used to produce antibodies

Glycoconjugates

Carrier Name

Keyhole limpet hemocyanin

Conjugation Method

The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage

Antibodies

Antisera

Antibody type and class

N/A

Assay System

ELISA, phagocytosis assays and protection experiments

Cross-reactivity

This antisera cross-reacted with capsular polysaccharide of 6A strains

Proposed epitopes

N/A

Proposed Utility

This antisera could passively protect mice against a 6B and 6A challenge

Weblink

PubMed Central

External Links

• Link to CarboDB database