PolysacDB Capsular polysaccharide 1012
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:02, 8 April 2010
Carbohydrate Name
Capsular polysaccharide
Carbohydrate Class
Capsular polysaccharide
Source Microbe
Streptococci Group B
Basic Structure
A basic backbone of the following residues: -3-β-D-Galp-(1-->4) -β-D-Glcp[branched to 1-->4-D-Glp-α-D-NeuNACp]-(1-->6)-β-D-GlcNAcp-1-
Proposed functions
Invades the blood stream and multiply. This property of invasiveness is related to the anti-phagocytic properties conferred by its Capsular polysaccharide
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Tetanus toxoid
Conjugation Method
Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4C in a pH stat. Adipic acid dihydrazide [AH] was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. After tumbling for 18 h at 3 to 8C, the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8C and passed through a 4B-CL Sepharose column. The polysaccharide-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and tetanus toxoid [TT} per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M, and the pH was maintained at 5.6 with 0.1 N NaOH for 3 h at room temperature. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8C and was passed through a 4B-CL Sepharose column (5 by 95 cm) equilibrated in 0.2 M NaCl. The void volume fractions were stored in 0.01% thimerosal at 3 to 8C
Antibodies
Polysera
Antibody type and class
mainly IgG, particularly IgG1 and IgG3
Assay System
Double immunodiffusion, capillary precipitation, ELISA
Cross-reactivity
N/A
Proposed epitopes
N\A
Proposed Utility
Antibodies elicited by the conjugates had in vitroopsonic activities proposed to be a correlate of protective immunity
