PolysacDB Beta-glucan 1015
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:02, 8 April 2010
Carbohydrate Name
Beta-glucan
Carbohydrate Class
Miscellaneous
Source Microbe
Candida albicans
Basic Structure
Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
Proposed functions
Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Diphtheria toxoid CRM 197
Conjugation Method
A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices
Antibodies
Polysera
Antibody type and class
IgG
Assay System
invivo protection assays, growth inhibition assays, indirect ELISA
Cross-reactivity
N/A
Proposed epitopes
N\A
Proposed Utility
The Lam-CRM conjugate is immunogenic and protective against systemic candidiasis in mice. The antibodies induce passive protection in mice against systemic and mucosal candidiasis
