PolysacDB Beta-glucan 1015

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1 Carbohydrate Name
2 Carbohydrate Class
3 Source Microbe
4 Basic Structure
5 Proposed functions
6 Antigenic Nature used to produce antibodies
7 Carrier Name
8 Conjugation Method
9 Antibodies
10 Antibody type and class
11 Assay System
12 Cross-reactivity
13 Proposed epitopes
14 Proposed Utility
15 Weblink
16 External Links

[edit] Carbohydrate Name

Beta-glucan

[edit] Carbohydrate Class

Miscellaneous

[edit] Source Microbe

Candida albicans

[edit] Basic Structure

Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length

[edit] Proposed functions

Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability

[edit] Antigenic Nature used to produce antibodies

Glycoconjugates

[edit] Carrier Name

Diphtheria toxoid CRM 197

[edit] Conjugation Method

A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices

[edit] Antibodies

Polysera

[edit] Antibody type and class

IgG

[edit] Assay System

invivo protection assays, growth inhibition assays, indirect ELISA

[edit] Cross-reactivity

N/A

[edit] Proposed epitopes

N\A

[edit] Proposed Utility

The Lam-CRM conjugate is immunogenic and protective against systemic candidiasis in mice. The antibodies induce passive protection in mice against systemic and mucosal candidiasis