PolysacDB 1448
From DrugPedia: A Wikipedia for Drug discovery
(Difference between revisions)
Revision as of 06:08, 8 April 2010
Carbohydrate Name
Capsular polysaccharide [heteroglycan]
Carbohydrate Class
Capsular polysaccharide
Source Microbe
Enterococcus faecalis type V
Basic Structure
It is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
Proposed functions
These polysaccharides are major virulence factors
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Tetanus toxoid
Conjugation Method
PSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH3
Antibodies
Polysera
Antibody type and class
N/A
Assay System
Competitive inhibition ELISA
Cross-reactivity
This antibody cross-reacted with E. faecalis strain 68114, 324057B and R19001. did not cross-react with E. faecalis type II PS and vancomycin-resistant E. faecium B210860
Proposed epitopes
N/A
Proposed Utility
N/A
