PolysacDB Lipopolysaccharide 2312
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:13, 8 April 2010
Carbohydrate Name
Lipopolysaccharide
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Shigella flexneri serotype 5a
Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
mIgA
Antibody type and class
IgA
Assay System
ELISA, In Vivo Protection Experiments
Cross-reactivity
The antibody recognized 5a LPS but failed to react with 2a LPS and Y LPS
Proposed epitopes
mlgA was directed against a serotype specific epitope comprising at least the glucosyl residue specifically linked to the central rhamnose of the basic structure of the S. flexneri LPS
Proposed Utility
This Mab protected the animals against subsequent intranasal challenge with S. flexneri serotype 5a
