PolysacDB O-polysaccharide 2319
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Revision as of 06:13, 8 April 2010
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Shigella flexneri serotype 1b
Basic Structure
Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
Proposed functions
LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodies
Heat killed cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab MASF I
Antibody type and class
IgG1
Assay System
immunoblotting, enzyme-linked immunosorbent assay (ELISA), hemagglutination, and coagglutination
Cross-reactivity
The type I O antigen-specific antibodies were shown to bind exclusively to S. flexneri serotypes la and lb in ELISA
Proposed epitopes
The α-D-Glcp-(1-->4)-β-D-GlcpNAc disaccharide in the repeating unit of the O-antigen is the immunodominant region to which these antibodies bind
Proposed Utility
This Mab can be used for detection and identification of shigella flexneri species
