PolysacDB 2472
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:13, 8 April 2010
Carbohydrate Name
Excretory-secretory antigen [ES]
Carbohydrate Class
Miscellaneous
Source Microbe
Toxocara canis
Basic Structure
Excretory-secretory (ES) antigens of this species are composed of a restricted range of components, with overlapping but not identical profiles. The ES antigens range in m.w. from 32,000 (32 KDa) to 400 KDa and include at least one allergenic component. They are each glycoproteins, containing in all 40% by weight of carbohydrate, and differential lectin binding and protease sensitivity indicate that the major components are discrete parasite antigens rather than degradation products of common derivation
Proposed functions
N/A
Antigenic Nature used to produce antibodies
T. canis larvae
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab Tcn-1
Antibody type and class
IgG1
Assay System
ELISA, immunoblotting, immunofluorescence, Immunoradiometric assay and inhibition assay
Cross-reactivity
This Mab cross-reacted and showed positive reactions to surface and ES antigens of T. cati, but did not react with surface-labeled antigens of the nematodes T.spiralis, Heligmososmoides polygyrus. or B. pahangi
Proposed epitopes
N/A
Proposed Utility
This Mab has been used to determine which epitopes are exposed and which are inaccessible to the parasite surface. These probes indicate that a major parasite antigen is not expressed, or exposed, on the larval surface on emergence from the infective ovum. but subsequently takes its place as the most prominent surface antigen
