PolysacDB O-polysaccharide 2499
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:14, 8 April 2010
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Vibrio cholerae O1 LPS serotype ogawa 395
Basic Structure
The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar in the Inaba O-antigen has a hydroxyl at the 2' position
Proposed functions
V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab 2D6
Antibody type and class
IgA
Assay System
ELISA, agglutination assay, indirect immunofluorescence, EM immunogold labeling, invivo protection and immunoblotting
Cross-reactivity
This Mab is specific to V. cholerae Ogawa and did not cross-react with V. cholerae CA401, a virulent strain of the Inaba serotype
Proposed epitopes
The nonreducing terminal residue of Ogawa O-PS is the dominant determinant. The C-2 O-methyl group fits into a somewhat flexible antibody cavity and that hydrogen bonds involving the oxygen and, respectively, the OH at the 2- and 3-position of the sugar moiety as well as the 2'-position in the amide side chain are required. [9446585]
Proposed Utility
Neonatal mice bearing anti-lipopolysaccharide hybridoma backpack tumors were specifically protected against oral challenge
