PolysacDB O-polysaccharide 2564
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Revision as of 06:14, 8 April 2010
Carbohydrate Name
O-polysaccharide
Carbohydrate Class
O-polysaccharide [LPS]
Source Microbe
Xanthomonas hyacinthi S148
Basic Structure
The Xanthomonas LPS showed a predominance of mannose (Man), glucose (Glc), galacturonic acid (GalA), and N-acetylglucosamine (GlcNAc) residues
Proposed functions
N/A
Antigenic Nature used to produce antibodies
crude cell wall preparation and purified fimbriae
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab 2E5
Antibody type and class
IgM
Assay System
immunoblotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy
Cross-reactivity
This Mab cross-reacted with all X. hyacinthi strains. No cross-reaction was found with 27 other Xanthomonas pathovars tested or with 14 other bacterial species from other genera, such as Erwinia and Pseudomonas
Proposed epitopes
The O antigen was found to consist of rhamnose and fucose in a 2:1 molar ratio. Mab 2E5 recognized only S-LPS and did not recognize R-LPS
Proposed Utility
This Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes
