PolysacDB Lipid-A 2574
From DrugPedia: A Wikipedia for Drug discovery
Revision as of 06:14, 8 April 2010
Carbohydrate Name
Lipid-A
Carbohydrate Class
Lipopolysaccharide
Source Microbe
Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety
Basic Structure
Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall
Proposed functions
Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment
Antigenic Nature used to produce antibodies
Whole cells
Carrier Name
nil
Conjugation Method
nil
Antibodies
Mab JIM32
Antibody type and class
IgG2b
Assay System
dot immunoassay, immunoblotting
Cross-reactivity
This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3. JIM 32 reacted positively with the LPS from many strains of Rhizobium tested (excluding the Rhizobium meliloti subgroup). JIM 32 did not react with representative strains from Bradyrhizobium, Azorhizobium or other related bacterial species
Proposed epitopes
galacturonic acid residues seemed to be a part of the immunodominanat region
Proposed Utility
This Mab could be useful for the elucidation of the structure and biosynthesis of LPS
