PolysacDB Lipid-A 2580
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Lipid-A
[edit] Carbohydrate Class
Lipopolysaccharide
[edit] Source Microbe
Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety
[edit] Basic Structure
Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall
[edit] Proposed functions
Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment
[edit] Antigenic Nature used to produce antibodies
Whole cells
[edit] Carrier Name
nil
[edit] Conjugation Method
nil
[edit] Antibodies
Mab JIM38
[edit] Antibody type and class
IgM
[edit] Assay System
dot immunoassay, immunoblotting
[edit] Cross-reactivity
This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3
[edit] Proposed epitopes
galacturonic acid residues seemed to be a part of the immunodominanat region
[edit] Proposed Utility
This Mab could be useful for the elucidation of the structure and biosynthesis of LPS
