From DrugPedia: A Wikipedia for Drug discovery

Immunotation of Rv0013
Name
POSSIBLE ANTHRANILATE SYNTHASE COMPONENT II TRPG (GLUTAMINE AMIDOTRANSFERASE)
Identifiers
Swiss Prot	Q7DAK6
Genbank	885955
PDB	?
Chemical data
Formula	?
Mol. wt.	24626.9 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	6.502

Contents 1 General 1.1 Protein Sequence 2 Human Homologue Blast Result 3 Alergen Protein 4 Bacterial Toxin Prediction 5 Subcellular Location 6 Antigens 6.1 B cell Epitopes 6.1.1 BCEpred Analysis 6.1.2 ABCpred Analysis 6.1.3 IEDB Analysis 7 MHC Class-I Binder 7.1 NHLA Analysis 7.2 IEDB Analysis 8 MHC Class-II Binder 8.1 Propred Analysis 8.2 NetMHC-II Analysis

General

Mb0013, trpG, len: 232 aa. Equivalent to Rv0013, len: 232 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 232 aa overlap). Possible trpG, anthranilate synthase component II (glutamine amidotransferase) (EC 4.1.3.27), equivalent to NP_301141.1|NC_002677 putative p-aminobenzoate synthase glutamine amidotransferase from Mycobacterium leprae (232 aa). Also highly similar to others e.g. P26922|TRPG_AZOBR Anthranilate synthase component II from Azospirillum brasilense (196 aa), FASTA scores: opt: 703, E(): 8.6e-40, (56.7% identity in 187 aa overlap); T36720 probable glutamine amidotransferase from Streptomyces coelicolor (212 aa); T44524 anthranilate synthase from Nitrosomonas europaea (199 aa); etc. Also similar to E235740 para-aminobenzoate synthase (232 aa), FASTA scores: opt: 1273, E(): 0, (79.7% identity in 232 aa overlap). Contains PS00606 Beta-ketoacyl synthases active site; and PS00442 Glutamine amidotransferases class-I active site. SIMILARITY TO OTHER TYPE-1 GLUTAMINE AMIDOTRANSFERASE DOMAINS. Note that previously known as pabA.

MOLECULAR FUNTION

POSSIBLY INVOLVED IN BIOSYNTHESIS OF TRYPTOPHAN (AT THE FIRST STEP). SUPPOSED TETRAMER OF TWO COMPONENTS I AND TWO COMPONENTS II: COMPONENT I (Rv1609|trpE) CATALYZES THE FORMATION OF ANTHRANILATE USING AMMONIA RATHER THAN GLUTAMINE, WHEREAS COMPONENT II (Rv0013|trpG) PROVIDES GLUTAMINE AMIDOTRANSFERASE ACTIVITY. POSSIBLY PARTICIPATES IN THE TRYPTOPHAN-DEPENDENT INDOLE-3-ACETIC ACID PRODUCTION [CATALYTIC ACTIVITY: CHORISMATE + L-GLUTAMINE = ANTHRANILATE + PYRUVATE + L-GLUTAMATE].

Protein Sequence

>M. bovis AF2122/97|Mb0013|TrpG: 232 aa - POSSIBLE ANTHRANILATE SYNTHASE COMPONENT II TRPG (GLUTAMINE AMIDOTRANSFERASE)</b>

MRILVVDNYDSFVFNLVQYLGQLGIEAEVWRNDDHRLSDEA AVAGQFDGVLLSPGPGTPERAGASVSMVHACAAAHTPLLGV CLGHQAIGVAFGATVDRAPELLHGKTSSVFHTNVGVLQGLP DPFTATRYHSLTILPKSLPAVLRVTARTSSGVIMAVQHTGL PIHGVQFHPESILTEGGHRILANWLTCCGWTQDDTLVRRLE NEVLTAISPHFPTSTASAGEATGRTSA

Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp|P27708.3|	29	46	132	1e-08
sp|P49915.1|	28	44	112	2e-06
sp|P31327.2|	27	42	137	2e-05
sp|Q92820.2|	24	40	123	0.33
sp|Q6UW60.1|	28	40	75	5.5

Alergen Protein

Link to Algpred
Non Allergen Predicted by AlgPred Server

Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

Subcellular Location

Link to TBpred
Cytoplasmic Protein

Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

B cell Epitopes

BCEpred Analysis

Link to Bcepred  
Result Predicited by[1]

ABCpred Analysis

Link to ABCpred
Result Predicted By [2]

IEDB Analysis

Link to IEDB
No Hits Found.

MHC Class-I Binder

NHLA Analysis

Link to [3]
Results[4]</br>

IEDB Analysis

Link to IEDB
Results[5]

MHC Class-II Binder

Propred Analysis

Link to Propred
Results[6]

NetMHC-II Analysis

Link to NetMHC-II
Results