CarboDB Glycopeptidolipid antigen 1671

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1 Carbohydrate Name
2 Carbohydrate Class
3 Source Microbe
4 Basic Structure
5 Proposed functions
6 Antigenic Nature used to produce antibodies
7 Carrier Name
8 Conjugation Method
9 Antibodies
10 Antibody type and class
11 Assay System
12 Cross-reactivity
13 Proposed epitopes
14 Proposed Utility
15 Weblink
16 External Links

Carbohydrate Name

Glycopeptidolipid antigen

Carbohydrate Class

Miscellaneous

Source Microbe

Mycobacterium avium complex serovar 8

Basic Structure

This antigen is composed of a glycosylated lipopeptide core, fatty acyl-D-phenylalanine-D-allothreonine-D-alanine-L-alaninyl-O-(3,4-di-O-methyl-α-L-rhamnopyranoside), to which a haptenic oligosaccharide is linked at the threonine substituent

Proposed functions

This oligosaccharide unit is the source of the dominant immunogenicity of members of the M. avium complex, i.e., their characteristic agglutinability and type specificity

Antigenic Nature used to produce antibodies

whole cells combined with whole unfractionated lipid

Carrier Name

nil

Conjugation Method

nil

Antibodies

Mab 5F7

Antibody type and class

IgG3

Assay System

ELISA, Inhibition assays, dot blot

Cross-reactivity

This antibody cross-reacted with whole cells of serovar 8, acetylated-GPL-8, and GPL-8

Proposed epitopes

4,6-(l'-carboxyethylidene)-3-0-methyl-beta-D-glucopyranosyl residues seemed to be a part of the epitope

Proposed Utility

This Mab can be used for the identification and classification of M. avium isolates. This Mab can be applied directly to bacterial cultures, thus allowing easy, rapid identification of at least half of the M. avium isolates encountered in the clinical laboratory