CarboDB Tetrasaccharide 2001
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Revision as of 00:00, 15 January 2001 by Special:Contributions/ (Talk)
Carbohydrate Name
Tetrasaccharide
Carbohydrate Class
Capsular polysaccharide
Source Microbe
Streptococcus pneumoniae serotype 6B
Basic Structure
The structure of the trisaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
Proposed functions
N/A
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Keyhole limpet hemocyanin
Conjugation Method
The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
Antibodies
Antisera
Antibody type and class
N/A
Assay System
ELISA, phagocytosis assays and protection experiments
Cross-reactivity
This antisera cross-reacted with capsular polysaccharide of 6A strains
Proposed epitopes
N/A
Proposed Utility
This antisera could passively protect mice against a 6B and 6A challenge
