From DrugPedia: A Wikipedia for Drug discovery

Rv0189c
Name
DIHYDROXY-ACID DEHYDRATASE (DAD) (ILVD)
Identifiers
Swiss Prot	P65154
Genbank	886774
PDB	?
Chemical data
Formula	C13H13N3OS [1]
Mol. wt.	59353 Da
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	4.8

Contents 1 General 1.1 Protein Sequence 2 Human Homologue Blast Result 3 Alergen Protein 4 Bacterial Toxin Prediction 5 Subcellular Location 6 Antigens 6.1 B cell Epitopes 6.1.1 BCEpred Analysis 6.1.2 ABCpred Analysis 6.1.3 IEDB Analysis 7 MHC Class-I Binder 7.1 nHLAPred Analysis 7.2 IEDB Analysis 8 MHC Class-II Binder 8.1 Propred Analysis 8.2 NetMHC-II Analysis 9 External Links

General

INVOLVED IN VALINE AND ISOLEUCINE BIOSYNTHESIS (AT THE FOURTH STEP) [CATALYTIC ACTIVITY: 2,3-DIHYDROXY-3-METHYLBUTANOATE = 3-METHYL-2- OXOBUTANOATE + H(2)O]. essential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003) mutants available at TARGET website

Protein Sequence

>Rv0189c, TB.seq 219997:221721 MW:59353 MPQTTDEAASVSTVADIKPRSRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLANAVKEGVF SAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYA GSILPGRAKLSDGSERDVTIIDAFEAVGACSRGLMSRADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAP PATDRRRDGFARRSGQAVVELLRRGITARDILTKEAFENAIAVVMAFGGSTNAVLHLLAIAHEANVALSLQDFSRIGSGV PHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAENLAAITPPDPDGKVLRALANPIHPSGGITI LHGSLAPEGAVVKTAGFDSDVFEGTARVFDGERAALDALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGK DVLLLTDGRFSGGTTGLCVGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFASRQQDFSPPPPRYTTGVLSK YVKLVSSAAVGAVCG

Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp|P52701.2	26	47	53	3.4
sp|P08034.1	24	44	93	3.7
sp|Q9HBR0.2	41	56	39	3.7
sp|Q9Y2L5.1	19	36	205	6.1
sp|P46926.1	30	39	113	6.7

Alergen Protein

Link to Algpred
Non Allergen Predicted by AlgPred Server

Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

Subcellular Location

Link to TBpred
Cytoplasmic

Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

B cell Epitopes

BCEpred Analysis

Link to Bcepred
Result Predicited by [2]

ABCpred Analysis

Link to ABCpred
Result Predicited by [3]

IEDB Analysis

Link to IEDB
Result Predicited by [4]

MHC Class-I Binder

nHLAPred Analysis

Link to nHLApred
Result Predicited by [5]

IEDB Analysis

Link to IEDB
Result Predicted by [6]

MHC Class-II Binder

Propred Analysis

Link to Propred
Result Predicted by [7]

NetMHC-II Analysis

Link to NetMHC-II
Result Predicted by [8]

External Links

• Database of Mycobacterium tuberculosis genome sequences and related information.