Protein Refolding

From DrugPedia: A Wikipedia for Drug discovery

Recombinant DNA technology allows the expression of valuable heterologous proteins at high expression rates. Particularly in Escherichia coli (E. coli) overexpression of proteins often leads to aggregation and deposition in dense, insoluble particles within the host cell, so-called inclusion bodies (IB). Formation of inclusion bodies is heavily protein dependent, charge distribution and turn forming residues have a strong impact (1),