PolysacDB Core oligosaccharide 2576

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Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Core oligosaccharide

[edit] Carbohydrate Class

Core oligosaccharide [LPS]

[edit] Source Microbe

Mutant derivatives of Rhizobium leguminosarum 3841 with LPS structures lacking the major O-antigen moiety

[edit] Basic Structure

Two different classes (LPS-1 and LPS-2) have been defined. LPS-1 macromolecules are formed from three components, i.e., lipid A, core oligosaccharide, and O-antigen, whereas LPS-2 lacks the O-antigen. Lipid A is normally anchored in the outer side of the outer membrane. In R. leguminosarum, the carbohydrate moiety of lipid A is composed of galacturonic acid, glucosamine, and amino-deoxygluconic acid. These molecules are acylated with diverse hydroxy fatty acids of variable lengths (C14 to C18). There is also a unique 27-hydroxy-octacosanoic acid (27-OH C28:0) which could span the whole lipid bilayer. The core oligosaccharide component is structurally conserved among strains and is built from a trisaccharide and a tetrasaccharide, with galacturonic acid, mannose, galactose, and 3-deoxy-D-mannooctulonic acid constituents. The O-antigen region is composed of a strain-specific oligosaccharide repeating unit which projects beyond the outer membrane and is the most antigenic component of the cell wall

[edit] Proposed functions

Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment

[edit] Antigenic Nature used to produce antibodies

Whole cells

[edit] Carrier Name

nil

[edit] Conjugation Method

nil

[edit] Antibodies

Mab JIM34

[edit] Antibody type and class

IgM

[edit] Assay System

dot immunoassay, immunoblotting

[edit] Cross-reactivity

This Mab cross-reacted with LPS from strain CE109, a derivative of Rhizobium etli CE3

[edit] Proposed epitopes

galacturonic acid residues seemed to be a part of the immunodominanat region

[edit] Proposed Utility

This Mab could be useful for the elucidation of the structure and biosynthesis of LPS

[edit] Weblink

PubMed Central

[edit] External Links

• Link to PolysacDB database