PolysacDB 1449
From DrugPedia: A Wikipedia for Drug discovery
[edit] Carbohydrate Name
Capsular polysaccharide [heteroglycan]
[edit] Carbohydrate Class
Capsular polysaccharide
[edit] Source Microbe
Enterococci faecium B210860
[edit] Basic Structure
It is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
[edit] Proposed functions
These polysaccharides are major virulence factors
[edit] Antigenic Nature used to produce antibodies
Glycoconjugates
[edit] Carrier Name
Tetanus toxoid
[edit] Conjugation Method
PSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH4
[edit] Antibodies
Polysera
[edit] Antibody type and class
N/A
[edit] Assay System
Competitive inhibition ELISA
[edit] Cross-reactivity
This antibody was specific to E. faecium B210860 only and did not cross-react with polysaccharides from E. faecalis type II, 68114, and R19001
[edit] Proposed epitopes
N/A
[edit] Proposed Utility
N/A
