Immunotation of Rv0084

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{{immunebox
{{immunebox
-
| Name        =<b> Dihydrodipicolinate Synthase</b>
+
| Name        =<b> POSSIBLE FORMATE HYDROGENLYASE HYCD (FHL)</b>
|image=
|image=
| width=250
| width=250
|image2=
|image2=
-
|Swiss Prot =  P63945
+
|Swiss Prot =  Q10881
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| Genbank          = 888289
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| Genbank          = 886959
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| PDB        = 1XXX
+
| PDB        =
| DrugBank          =
| DrugBank          =
-
| chemical_formula  = C28H37ClN4O6 [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=18062777&loc=ec_rcs#Properties]
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| chemical_formula  = [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=7711&loc=ec_rcs]
-
| molecular_weight  = 30,858 Da
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| molecular_weight  = 32152.9 Da
| solubility        =  
| solubility        =  
-
|Isoelectric-Point = 5.57
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|Isoelectric-Point = 6.52
}}
}}
-
'''Protein Sequence'''
+
=General=
 +
Rv0084, (MTCY251.02), len: 316 aa. Possible hycD (alternate gene name: hevD), formate hydrogenlyase  integral membrane protein, similar to others e.g. HYCD_ECOLI|P16430 formate hydrogenlyase subunit 4 from Escherichia coli (307 aa).Also similar to NUOH_ECOLI|P33603 NADH dehydrogenase I chain H from Escherichia coli (325 aa), FASTA scores: opt: 207, E(): 9.5e-06, (26.5% identity in 260 aa overlap). BELONGS TO THE COMPLEX I SUBUNIT 1 FAMILY; hevD
 +
 
 +
 
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'''POSSIBLE MOLECULAR FUNTION'''
 +
 
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1.LYASE ACTIVITY
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Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but only one in the other direction. When acting on the single substrate, a molecule is eliminated and this generates either a new double bond or a new ring.
 +
 
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2.OXIDOREDUCTASE ACTIVITY
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Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
 +
 
 +
 
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==Protein Sequence==
>Rv0084, TB.seq  92326:93273 MW:32154  
>Rv0084, TB.seq  92326:93273 MW:32154  
Line 25: Line 38:
LALLANLFLPWGIAGAAPTALDVLTGVVAVAAKVAILAVLLATFEVF
LALLANLFLPWGIAGAAPTALDVLTGVVAVAAKVAILAVLLATFEVF
LAKLRLFRVPELLAGSFLLALLAVTAANFFTVGA
LAKLRLFRVPELLAGSFLLALLAVTAANFFTVGA
 +
 +
=Human Homologue Blast Result=
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<table border='1'><tr>
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<td>subject ids</td><td>% identity</td><td>% positives</td><td>alignment length</td><td> evalue</td></tr>
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<tr><td>sp|P03886.1|</td><td> 25</td><td> 47</td><td> 318</td><td> 0.045</td></tr>
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<tr><td>sp|Q709C8.1|</td><td> 23</td><td> 42</td><td> 3753</td><td>  3.9</td></tr>
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<tr><td>sp|Q53EL9.2|</td><td> 28</td><td> 47</td><td> 994</td><td>    3.9</td></tr></table>
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 +
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=Alergen Protein=
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Link to [http://www.imtech.res.in/raghava/algpred/ Algpred]<br>
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Non Allergen Predicted by AlgPred Server
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=Bacterial Toxin Prediction=
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Link to [http://www.imtech.res.in/raghava/btxpred/submission.html btxpred]<br>
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No Hit Fountd by btxpred server.
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=Subcellular Location=
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Link to [http://www.imtech.res.in/raghava/tbpred/ TBpred]<br>
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Integral Membrane Protein
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=Antigens=
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No Hit Found in [http://www.imtech.res.in/raghava/antigendb/keyquery.html AntigenDB]<br>
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No. Hit Found in [http://www.iedb.org/ IEDB]
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==B cell Epitopes==
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====BCEpred Analysis====
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Link to [http://www.imtech.res.in/raghava/bcepred/bcepred_submission.html Bcepred]  
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<br>
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Result Predicited by  [http://crdd.osdd.net/drugpedia/index.php/Image:BcePred_Prediction_Serverresults1.pdf]<br>
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====ABCpred Analysis====
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Link to [http://www.imtech.res.in/raghava/abcpred/ABC_submission.html ABCpred]<br>
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Result Predicted By
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[http://crdd.osdd.net/drugpedia/index.php/Image:ABCpred_Prediction_Serverresults1.pdf]<br>
 +
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====IEDB Analysis====
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Link to [http://tools.immuneepitope.org/tools/bcell/iedb_input IEDB]<br>
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Result Predicited by  [http://crdd.osdd.net/drugpedia/index.php/Image:IEDB_Results.pdf]<br>
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=MHC Class-I Binder=
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==NHLA Analysis==
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Link to [http://www.imtech.res.in/raghava/nhlapred/comp.html]<br>
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Results[http://crdd.osdd.net/drugpedia/index.php/Image:NHLAPredresults.doc]<br>
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==IEDB Analysis==
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Link to [http://tools.immuneepitope.org/analyze/html/mhc_processing.html IEDB] <br>
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Results[http://crdd.osdd.net/drugpedia/index.php/Image:MHC-I_results_IEDB.doc]<br>
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=MHC Class-II Binder=
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==Propred Analysis==
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Link to [http://www.imtech.res.in/raghava/propred/ Propred]<br>
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Results[http://crdd.osdd.net/drugpedia/index.php/Image:MHC_Class-II_Prophed_results.doc]<br>
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==NetMHC-II Analysis==
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Link to [http://www.cbs.dtu.dk/services/NetMHCII/ NetMHC-II]<br>
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Results[http://crdd.osdd.net/drugpedia/index.php/Image:NetMHCII_2_Results.doc]<br>
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=External Links=
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* [http://www.biohealthbase.org/mycobacterium Database] of ''Mycobacterium tuberculosis'' genome sequences and related information.
 +
 +
[[Category:C2D]]
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[[Category:Mtb Immunotation]]

Current revision

Immunotation of Rv0084
Name
POSSIBLE FORMATE HYDROGENLYASE HYCD (FHL)
Identifiers
Swiss Prot Q10881
Genbank 886959
PDB  ?
Chemical data
Formula [1]
Mol. wt. 32152.9 Da
Pharmacokinetic data
Bioavailability  ?
Solubility  ?
Isoelectric-Point 6.52

Contents

[edit] General

Rv0084, (MTCY251.02), len: 316 aa. Possible hycD (alternate gene name: hevD), formate hydrogenlyase integral membrane protein, similar to others e.g. HYCD_ECOLI|P16430 formate hydrogenlyase subunit 4 from Escherichia coli (307 aa).Also similar to NUOH_ECOLI|P33603 NADH dehydrogenase I chain H from Escherichia coli (325 aa), FASTA scores: opt: 207, E(): 9.5e-06, (26.5% identity in 260 aa overlap). BELONGS TO THE COMPLEX I SUBUNIT 1 FAMILY; hevD


POSSIBLE MOLECULAR FUNTION

1.LYASE ACTIVITY Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but only one in the other direction. When acting on the single substrate, a molecule is eliminated and this generates either a new double bond or a new ring.

2.OXIDOREDUCTASE ACTIVITY Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.


[edit] Protein Sequence

>Rv0084, TB.seq 92326:93273 MW:32154


MSYLAGAAQIGGVMVGAPLVIGMTRQVRARWEGRAGAGLLQPWRDLL KQLGKQQITPAGTTIVFAAAPVIVAGTTLLIAAIAPLVATGSPLDPS ADLFAVVGLLFLGTVALTLAGIDTGTSFGGMGASREITIAALVEPTI LLAVFALSIPAGSANLGALVASTIDHPGHVVSLAGVLAFVALVIVIV AETGRLPVDNPATHLELTMVHEAMVLEYAGPRLALVEWAAGMRLTVL LALLANLFLPWGIAGAAPTALDVLTGVVAVAAKVAILAVLLATFEVF LAKLRLFRVPELLAGSFLLALLAVTAANFFTVGA

[edit] Human Homologue Blast Result

subject ids% identity% positivesalignment length evalue
sp|P03886.1| 25 47 318 0.045
sp|Q709C8.1| 23 42 3753 3.9
sp|Q53EL9.2| 28 47 994 3.9


[edit] Alergen Protein

Link to Algpred
Non Allergen Predicted by AlgPred Server

[edit] Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

[edit] Subcellular Location

Link to TBpred
Integral Membrane Protein

[edit] Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

[edit] B cell Epitopes

[edit] BCEpred Analysis

Link to Bcepred  
Result Predicited by [2]

[edit] ABCpred Analysis

Link to ABCpred
Result Predicted By [3]

[edit] IEDB Analysis

Link to IEDB
Result Predicited by [4]

[edit] MHC Class-I Binder

[edit] NHLA Analysis

Link to [5]
Results[6]

[edit] IEDB Analysis

Link to IEDB
Results[7]

[edit] MHC Class-II Binder

[edit] Propred Analysis

Link to Propred
Results[8]

[edit] NetMHC-II Analysis

Link to NetMHC-II
Results[9]

[edit] External Links

  • Database of Mycobacterium tuberculosis genome sequences and related information.
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