PolysacDB 1449

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Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

[edit] Carbohydrate Name

Capsular polysaccharide [heteroglycan]

[edit] Carbohydrate Class

Capsular polysaccharide

[edit] Source Microbe

Enterococci faecium B210860

[edit] Basic Structure

It is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine

[edit] Proposed functions

These polysaccharides are major virulence factors

[edit] Antigenic Nature used to produce antibodies

Glycoconjugates

[edit] Carrier Name

Tetanus toxoid

[edit] Conjugation Method

PSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH4

[edit] Antibodies

Polysera

[edit] Antibody type and class

N/A

[edit] Assay System

Competitive inhibition ELISA

[edit] Cross-reactivity

This antibody was specific to E. faecium B210860 only and did not cross-react with polysaccharides from E. faecalis type II, 68114, and R19001

[edit] Proposed epitopes

N/A

[edit] Proposed Utility

N/A

[edit] Weblink

PubMed Central

[edit] External Links

• Link to PolysacDB database