PolysacDB 1449
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Revision as of 06:08, 8 April 2010
Carbohydrate Name
Capsular polysaccharide [heteroglycan]
Carbohydrate Class
Capsular polysaccharide
Source Microbe
Enterococci faecium B210860
Basic Structure
It is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine
Proposed functions
These polysaccharides are major virulence factors
Antigenic Nature used to produce antibodies
Glycoconjugates
Carrier Name
Tetanus toxoid
Conjugation Method
PSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH4
Antibodies
Polysera
Antibody type and class
N/A
Assay System
Competitive inhibition ELISA
Cross-reactivity
This antibody was specific to E. faecium B210860 only and did not cross-react with polysaccharides from E. faecalis type II, 68114, and R19001
Proposed epitopes
N/A
Proposed Utility
N/A
