From DrugPedia: A Wikipedia for Drug discovery

Immunotation of Rv0032
Name
<Rv0032> POSSIBLE 8-AMINO-7-OXONONANOATE SYNTHASE BIOF2
Identifiers
Swiss Prot	P71602
Genbank	?
PDB	structure found no structure found
Chemical data
Formula	?
Mol. wt.	86243
Pharmacokinetic data
Bioavailability	?
Solubility	?
Isoelectric-Point	7.0184

Contents 1 General 1.1 Protein Sequence 2 Human Homologue Blast Result 3 Alergen Protein 4 Bacterial Toxin Prediction 5 Subcellular Location 6 Antigens 6.1 B cell Epitopes 6.1.1 BCEpred Analysis 6.1.2 ABCpred Analysis 6.1.3 IEDB Analysis 7 MHC Class-I Binder 7.1 nHLAPred Analysis 7.2 IEDB Analysis 8 MHC Class-II Binder 8.1 Propred Analysis 8.2 NetMHC-II Analysis 9 External Links

[edit] General

Rv0032, (MTCY10H4.32), len: 771 aa. Probable bioF2, 8-amino-7-oxononanoate synthase (EC 2.3.1.47), with its C-terminal similar to others e.g. BIOF_BACSU|P53556 8-amino-7-oxononanoate synthase from Bacillus subtilis (389 aa), FASTA scores: opt: 775, E(): 0, (37.9% identity in 346 aa overlap); P22806|BIOF_BACSH from Bacillus sphaericus (389 aa); etc. Also similar to BIOF1|Rv1569|MTCY336_35 from Mycobacterium tuberculosis (386 aa), AF041819_4 from Mycobacterium bovis, and BIOF_MYCLE|P45487 from Mycobacterium leprae (385 aa). Contains PS00599 Aminotransferases class-II pyridoxal-phosphate attachment site. BELONGS TO CLASS-II OF PYRIDOXAL-PHOSPHATE-DEPENDENT AMINOTRANSFERASES.

COULD BE INVOLVED IN BIOTIN BIOSYNTHESIS (AT THE FIRST STEP) [CATALYTIC ACTIVITY: 6-CARBOXYHEXANOYL-COA + L-ALANINE = 8-AMINO-7-OXONONANOATE + CoA + CO2].

[edit] Protein Sequence

>Rv0032, TB.seq 34295:36607 MW:86245 MPTGLGYDFLRPVEDSGINDLKHYYFMADLADGQPLGRANLYSVCFDLATTDRKLTPAWRTTIKRWFPGFMTFRFLECGL LTMVSNPLALRSDTDLERVLPVLAGQMDQLAHDDGSDFLMIRDVDPEHYQRYLDILRPLGFRPALGFSRVDTTISWSSVE EALGCLSHKRRLPLKTSLEFRERFGIEVEELDEYAEHAPVLARLWRNVKTEAKDYQREDLNPEFFAACSRHLHGRSRLWL FRYQGTPIAFFLNVWGADENYILLEWGIDRDFEHYRKANLYRAALMLSLKDAISRDKRRMEMGITNYFTKLRIPGARVIP TIYFLRHSTDPVHTATLARMMMHNIQRPTLPDDMSEEFCRWEERIRLDQDGLPEHDIFRKIDRQHKYTGLKLGGVYGFYP RFTGPQRSTVKAAELGEIVLLGTNSYLGLATHPEVVEASAEATRRYGTGCSGSPLLNGTLDLHVSLEQELACFLGKPAAV LCSTGYQSNLAAISALCESGDMIIQDALNHRSLFDAARLSGADFTLYRHNDMDHLARVLRRTEGRRRIIVVDAVFSMEGT VADLATIAELADRHGCRVYVDESHALGVLGPDGRGASAALGVLARMDVVMGTFSKSFASVGGFIAGDRPVVDYIRHNGSG HVFSASLPPAAAAATHAALRVSRREPDRRARVLAAAEYMATGLARQGYQAEYHGTAIVPVILGNPTVAHAGYLRLMRSGV YVNPVAPPAVPEERSGFRTSYLADHRQSDLDRALHVFAGLAEDLTPQGAAL

[edit] Human Homologue Blast Result

subject ids	% identity	% positives	alignment length	evalue
sp|O75600	32	50	344	3e-48
sp|P22557	32	55	325	3e-45
sp|Q9NUV7	30	50	378	4e-43
sp|O15270	31	50	356	9e-42
sp|P13196	31	50	338	2e-38

[edit] Alergen Protein

Link to Algpred
Non Allergen Predicted by AlgPred Server

[edit] Bacterial Toxin Prediction

Link to btxpred
No Hit Fountd by btxpred server.

[edit] Subcellular Location

Link to TBpred
CYTOPLASMIC PROTEIN

[edit] Antigens

No Hit Found in AntigenDB
No. Hit Found in IEDB

[edit] B cell Epitopes

[edit] BCEpred Analysis

Link to Bcepred
Result Predicited by Bcepred

[edit] ABCpred Analysis

Link to ABCpred
Result Predicited by ABCPred

[edit] IEDB Analysis

Link to IEDB
Result Predicited by IEDB

[edit] MHC Class-I Binder

[edit] nHLAPred Analysis

Link to nHLApred
Result Predicited by [1]

[edit] IEDB Analysis

Link to IEDB
Result Predicted by [2]

[edit] MHC Class-II Binder

[edit] Propred Analysis

Link to Propred
Result Predicted by [3]

[edit] NetMHC-II Analysis

Link to NetMHC-II
Result Predicted by []

[edit] External Links

• Database of Mycobacterium tuberculosis genome sequences and related information.