PolysacDB 1449

From DrugPedia: A Wikipedia for Drug discovery

Contents 1 Carbohydrate Name 2 Carbohydrate Class 3 Source Microbe 4 Basic Structure 5 Proposed functions 6 Antigenic Nature used to produce antibodies 7 Carrier Name 8 Conjugation Method 9 Antibodies 10 Antibody type and class 11 Assay System 12 Cross-reactivity 13 Proposed epitopes 14 Proposed Utility 15 Weblink 16 External Links

Carbohydrate Name

Capsular polysaccharide [heteroglycan]

Carbohydrate Class

Capsular polysaccharide

Source Microbe

Enterococci faecium B210860

Basic Structure

It is composed of rhamnose, glucose, galactose, mannosamine, and glucosamine

Proposed functions

These polysaccharides are major virulence factors

Antigenic Nature used to produce antibodies

Glycoconjugates

Carrier Name

Tetanus toxoid

Conjugation Method

PSs were oxidized with 0.5 ml of 10 mM NaI4 at room temperature for 90 min, dialyzed and lyophilized. The oxidized PS was reacted with 2 mg of tetanus toxoid in PBS with the addition of 10 mg of NaCNBH4

Antibodies

Polysera

Antibody type and class

N/A

Assay System

Competitive inhibition ELISA

Cross-reactivity

This antibody was specific to E. faecium B210860 only and did not cross-react with polysaccharides from E. faecalis type II, 68114, and R19001

Proposed epitopes

N/A

Proposed Utility

N/A

Weblink

PubMed Central

External Links

• Link to PolysacDB database